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Polyphenols, singlet oxygen & protein damage in the eye |
Specific Aims:
To determine the effect of specific botanical polyphenol preparations on the composition of polyphenols in lens and aqueous humor of the eye in a rat model using microfluidics analysis combined with mass spectrometry.
To determine the effects of polyphenols on the levels of the endogenous antioxidant glutathione, an exogenous antioxidant vitamins C and E, and carotenoids in specific regions of the eye.
To determine the effects of polyphenols on the extent of post-translational modifications and cross-linking of the lens a-crystallins in the rat model using 2D-electrophoresis and LC-tandem mass spectrometry.
To examine the mechanism of singlet oxygen-induced damage to a model protein (calmodulin) in an in vitro model system.
Project Leader:
Stephen Barnes PhD.
Co-Leader: Om Srivastava PhD.
Co-Investigator:
Chao-Cheng Wang PhD.
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